NAD+ 250MG is a coenzyme that participates in cellular metabolism. It is supplied in a lyophilized form for laboratory research. Each batch of NAD+ is verified for quality with HPLC and is manufactured in compliance with GMP standards. A specific Certificate of Analysis accompanies each batch. NAD+ is for Research Use Only (RUO).
Original price was: $ 140.$ 115Current price is: $ 115.
NAD+ 250MG is a research-grade coenzyme supplied in powder form for laboratory purposes only. This item is a freeze-dried (lyophilized) substance, and every lot is tested and certified for identity and potency by High-Performance Liquid Chromatography (HPLC). A Certificate of Analysis (CoA) is provided upon request for each purchased lot. NAD+ 250MG is categorized under BioPeak USA’s Cellular Science research area, alongside other materials used in metabolic and cellular signaling research.
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme found across virtually all living cells, where it participates in a wide range of enzymatic reactions central to cellular metabolism. Structurally, NAD+ is a dinucleotide — two nucleotide units joined through their phosphate groups, one carrying an adenine base and the other carrying a nicotinamide group. This structure is what allows NAD+ to function as an electron carrier, cycling between its oxidized form (NAD+) and its reduced form (NADH) during metabolic reactions.
In laboratory research, NAD+ is most commonly studied in the context of cellular energy metabolism, where it acts as a coenzyme in reactions such as glycolysis, the citric acid cycle, and oxidative phosphorylation — the core pathways cells use to convert nutrients into usable energy. Beyond its role as an electron carrier, NAD+ also serves as a substrate for several classes of enzymes, including sirtuins and PARPs (poly-ADP-ribose polymerases), both of which are active areas of ongoing cellular science research. Researchers investigating these pathways can review reference structural and chemical data for NAD+ (CAS 53-84-9) through PubChem.
| Specification | Detail |
|---|---|
| CAS Number | 53-84-9 |
| Format | Freeze-dried (lyophilized) powder, 250mg per vial |
| Purity | ≥99%, verified by HPLC on every batch |
| Documentation | Certificate of Analysis (CoA), batch-specific |
| Batch Testing | Every batch tested and documented individually |
| Intended Use | Research Use Only (RUO) |
| Testing Methods | HPLC, ESI-MS, Karl Fischer titration |
Before any batch leaves our facility, it goes through a full analytical workflow to confirm what it is, how pure it is, and how much residual moisture it contains. This layered approach reflects the same documentation standard applied across BioPeak USA’s broader Reference Standards line, where batch-specific verification is treated as a baseline requirement rather than an optional add-on.
This combination of methods reflects standard practice across the field of analytical chemistry — the National Institute of Standards and Technology (NIST) maintains extensive public resources on measurement science and analytical method validation relevant to this type of batch testing.
NAD+ has become a focal point of cellular science research over the past two decades, largely due to its central role connecting energy metabolism to a broader set of regulatory processes within the cell. Because NAD+ levels can shift measurably depending on metabolic state, researchers use it both as an experimental variable and as a readout for studying how cells respond to different conditions in a controlled in-vitro model.
Sirtuins — a family of NAD+-dependent enzymes — are one of the most actively studied downstream targets in this research area, since their enzymatic activity directly depends on NAD+ availability as a substrate. Similarly, PARP enzymes consume NAD+ during DNA repair-related processes, making NAD+ availability a relevant variable in laboratory models studying cellular stress response. Researchers investigating this literature can review peer-reviewed studies on NAD+ metabolism through PubMed.
Mostly for research into cellular energy metabolism and metabolic signaling, including studies involving sirtuin and PARP enzyme activity in controlled laboratory models.
Keep unopened vials at -20°C, as stated in the documentation, and minimize freeze-thaw cycling once reconstituted.
Yes. Every batch comes with a Certificate of Analysis showing purity and identity results, available through our COA Testing page.
At least 99% pure, confirmed by HPLC testing on every single batch, with identity independently confirmed by ESI-MS.
A Certificate of Analysis for that specific batch, covering purity, identity, and moisture content results.
No. NAD+ is the oxidized form of the coenzyme, while NADH is its reduced form. The two interconvert during metabolic reactions, and which form is relevant depends on the specific research question being studied.
Yes. NAD+ is frequently studied alongside other metabolic and signaling research materials available in BioPeak USA’s Cellular Science category.
NAD+ 250MG is a research compound that’s tested and documented batch by batch, so researchers know exactly what they’re working with. Its central role in cellular energy metabolism and its function as a substrate for sirtuins and PARP enzymes make it a widely studied coenzyme across cellular science research. Combined with dual-method analytical verification (HPLC and ESI-MS) and batch-specific documentation, it’s a solid choice for laboratories where quality traceability matters.
Researchers can also explore BioPeak USA’s Reference Standards and Molecular Science categories for related materials supplied with the same batch-specific documentation standard.
This product is sold strictly for laboratory and scientific research. It is not meant for human or animal use. It’s on the researcher to follow all applicable laws and their institution’s rules when handling it. By buying this product, you’re confirming you’re a qualified researcher and that you agree to BioPeak USA’s Terms of Service.
